To establish robust methodology using alternative tests to screen in vitro genotoxicity of MNs in pulmonary, oral and dermal cell systems. These assays will be applied to all MNs assessed. On the basis of the results obtained, a ring tests will be performed using the most promising approaches.
To generate in vitro genotoxicity data on MNs: Production of in vitro genotoxicity data on MNs using standard tests and modified assays utilizing specific cell models.
To perform a round robin test on in vitro testing of MNs : Based on in vitro genotoxicity and physical/chemical characterisation data obtained, a ring test on selected MNs will be carried out using the most promising in vitro assays
Several issues are still unclear in the in vitro genotoxicity testing of nanomaterials (MNs). The basic questions include (a) how well in vitro assays can be used for revealing the genotoxic potential of MNs, (b) which assays are suitable for this task, and (c) which modifications are needed in the commonly used tests when MNs are studied. WP5 aims at establishing a robust methodology using alternative tests to screen in vitro genotoxicity of MNs in pulmonary, oral and dermal cell systems (16 HBE, BEAS 2B, A549, Caco-2, reconstructed human epidermis, primary human lymphocytes etc.). These assays will be applied to all MNs assessed (only titanium dioxides will be tested in the dermal systems). On the basis of the results obtained, a round robin test will be performed using the most promising approach. During the first 12 months of the project, WP5 concentrated on setting up the in vitro methodology for the three categories of MNs (TiO2, SiO2 and carbon nanotubes) to be tested in WP5. The general principles of testing were agreed upon among WP5 partners. These concern for example: dose selection, treatment times, cytotoxicity assessment, negative and positive controls, specific details of the genotoxicity endpoints chosen ( DNA damage, micronuclei, and gene mutations), final choice of cell lines, and test protocols for each cell type. Details of the dispersion protocol provided by WP4 were discussed within WP5 and with WP4. Each laboratory tested the dispersion protocol and the ultrasonication devices available, according to WP4 instructions. The in vitro experimentation were started with cytotoxicity assessment used for dose finding, and the actual genotoxicity tests of the MNs begun
In vitro genotoxicity testing strategy for nanomaterials including database.
Associated partners working on WP5
Margarita Apostolova from the Institute of Molecular Biology "Roumen Tsenav" ( IMB-BAS, Bulgaria)
Wim de Jong, from the National Institute for Public Health and Environment ( RIVM, The Netherlands)
Valerie Fessard from French Food Safety Agency ( ANSES, France)
Mario Goetz from the Federal Institute for Risk Assessment ( BfR, Germany)
Yves Guichard from the Institut National de Recherche et de Sécurité ( INRS, France)
Ricardo Marcos, from the Universitat Autonoma de Barcelona ( UAB, Spain)
Fabrice Nesslany from the Insitut Pasteur of Lille ( IPL, France)
Maria Joao Silva from the National Health Institute Doutor Ricardo Jorge ( INSA, Portugal)
Maciej Stepnik from the Nofer Institute of Occupational Medicine ( NIOM, Poland)
Paul Troisfontaines from the Scientific Institute of Public Health ( IPH, Belgium)
Hakan Wallin from the National Research Centre for the Working Environment ( NRCWE, Denmark)
Collaborating partners working on WP5
Iseult Lynch from the University College Dublin ( UCD, Ireland)Last Updated on Thursday, 30 June 2011 11:23