12 August 2011
Some of the assay (lymphocytes micronucleus assay) provide indirect information about the potential toxicity of MNs.
The lymphocyte micronucleus assay was included because it has very much been used in regulatory testing and it is interesting to see how these cells perform in comparison with the cell lines used. Lymphocytes may not be very efficient in taking up MNs as compared with the other cell types used, but the smallest MNs are certainly expected to be internalized by lymphocytes too. Moreover, effects can also be mediated through action on the cellular membrane. It is a general question, concerning also other cell types, if much of the effects of MNs are actually indirect.